Long non-coding RNA Growth Arrest Specific 5 Regulates the Podocyte Function in Nephrotic Syndrome Development via microRNA-144-5p/Phosphatase And Tensin Homolog.

Researchers investigated how certain genetic molecules affect kidney function in people with nephrotic syndrome, a condition that causes the kidneys to leak protein into urine. The study focused on two molecules: a long non-coding RNA called GAS5 and a microRNA called miR-144-5p, which interact with each other in kidney cells called podocytes. Scientists found that patients with nephrotic syndrome had lower levels of GAS5 and higher levels of miR-144-5p compared to healthy individuals. Laboratory experiments showed that GAS5 helps protect kidney cells by reducing inflammation, oxidative stress, and cell death while promoting healthy cell growth. However, when miR-144-5p levels were high, it blocked these protective effects. The researchers also identified that GAS5 works by controlling miR-144-5p, which in turn affects a gene called PTEN that is important for maintaining healthy kidney cells. Combining measurements of both GAS5 and miR-144-5p in blood samples improved the ability to diagnose nephrotic syndrome. While this study does not directly involve acupuncture, understanding these molecular mechanisms may help explain how integrative approaches like acupuncture might support kidney health through reducing inflammation and oxidative stress, which are key factors this research identified. Patients interested in complementary approaches for kidney conditions should work with qualified acupuncturists experienced in treating renal disorders.

This study examined lncRNA GAS5 and miR-144-5p expression in primary nephrotic syndrome (PNS) patients and their regulatory mechanisms in podocyte function. RT-qPCR analysis revealed significantly decreased GAS5 and elevated miR-144-5p levels in PNS patients compared to controls. ROC analysis demonstrated improved diagnostic accuracy when combining both biomarkers. Dual-luciferase assays confirmed GAS5-miR-144-5p interaction. In vitro podocyte studies showed GAS5 overexpression reduced inflammatory markers, oxidative stress indices, and apoptosis while enhancing proliferation; miR-144-5p overexpression reversed these effects. Bioinformatics analysis identified PTEN and STAT3 as critical regulatory targets. The study suggests GAS5 exerts nephroprotective effects by sponging miR-144-5p, thereby modulating PTEN expression. Clinical relevance: These findings identify potential biomarkers for PNS screening and suggest therapeutic targets. From an integrative medicine perspective, interventions reducing inflammation and oxidative stress may complement conventional treatment approaches.