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Arthritis1 min read

[Effects of electroacupuncture on autophagy of the synovial tissue in the knee joint of rats with knee osteoarthritis by regulating PI3K/Akt/mTOR pathway].

Zhen ci yan jiu = Acupuncture researchยทJanuary 2026ยทXin-Hua Zhou, Qi-Qi Yang, Wei-Ran Li et al.
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Key Finding

Electroacupuncture at ST35 and ST36 significantly reduced synovial autophagy and improved knee joint function in osteoarthritic rats by activating the PI3K/Akt/mTOR signaling pathway.

What This Means For You

Researchers in China studied how electroacupuncture might help knee osteoarthritis by examining changes in knee joint tissue at the cellular level. They worked with 36 male rats divided into three groups: healthy rats, rats with induced knee arthritis, and rats with knee arthritis that received electroacupuncture treatment. The treatment group received electroacupuncture at two specific points on the leg (Stomach 35 and Stomach 36) for 20 minutes daily over three weeks. The study found that knee osteoarthritis triggered excessive autophagy, a cellular recycling process that, when overactive, can damage joint tissue. Rats with untreated arthritis showed increased inflammation, tissue damage, and poor knee function. However, rats receiving electroacupuncture showed significant improvements: their knee function scores improved, inflammation decreased, and tissue structure looked healthier under microscopic examination. The researchers discovered that electroacupuncture works by activating a specific cellular pathway called PI3K/Akt/mTOR, which helps regulate autophagy and reduce the excessive cellular recycling that damages joint tissue. The electroacupuncture treatment reduced markers of harmful autophagy while increasing protective signaling proteins. This suggests that electroacupuncture may help knee osteoarthritis by normalizing cellular processes in the joint lining, reducing inflammation, and improving overall joint health. While this animal study shows promising mechanisms, human clinical trials are needed to confirm these benefits in people with knee arthritis. If you're considering acupuncture for knee osteoarthritis, seek treatment from a licensed acupuncturist experienced in treating musculoskeletal conditions.

Clinical Notes for Practitioners

This animal study (n=36 male SD rats) investigated electroacupuncture's effect on synovial autophagy in sodium monoiodoacetate-induced knee osteoarthritis via PI3K/Akt/mTOR pathway modulation. EA was applied to ST35 and ST36 for 20 minutes daily over three weeks. Results showed significant improvements in the EA group compared to untreated controls: reduced Lequesne MG scores (P<0.01), decreased synovial inflammation and collagen proliferation, and reduced autophagosome/autolysosome numbers. Molecular analysis revealed EA significantly decreased autophagy markers (Atg5, Atg12, ULK1, Beclin-1, LC3II/LC3I ratio; P<0.01) while upregulating PI3K/Akt/mTOR pathway expression at both mRNA and protein levels (P<0.05-0.01). Findings suggest EA at ST35-ST36 inhibits excessive synovial autophagy through PI3K/Akt/mTOR activation, improving joint function and tissue morphology. This provides mechanistic evidence for EA's anti-inflammatory effects in KOA, supporting its clinical application for osteoarthritic knee conditions with a specific point prescription and treatment protocol validated at the molecular level.

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